The Genelily^TM m⁷G RNA Methylation Quantification Kit (Colorimetric) is suitable for detecting N7-methylguanosine (m⁷G) RNA methylation status directly using total RNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses.

Input RNA: The amount of RNA for each assay can be 100 ng to 300 ng. For optimal quantification, the input RNA amount should be 200 ng, as the 

abundance of m⁷G is generally less than 0.1% of total RNA.

Starting Material: Starting materials can include various tissue or cell samples such as cells from flask or microplate cultured cells, fresh and frozen tissues,

 paraffin-embedded tissues, blood, body fluid samples, etc.

Internal Control:Both negative and positive RNA controls are provided in this kit. A standard curve can be performed (range: 0.02 to 1 ng of m⁷G)or a single quantity of m⁷G can be used as a positive control. Because m⁷G content can vary from tissue to tissue, and from normal and diseased states,or vary under 

treated and untreated conditions, it is advised to run replicate samples to ensure that the signal generated is validated. This kit will allow the user to quantify an absolute amount of m⁷G and determine the relative m⁷G RNA methylation states of two different RNA samples.

试剂盒组成：

实验过程：

*本品仅供科研使用，请勿用于临床与诊断。