m6A RNA Methylation Quantification Kit(Colorimetric)

发布者：小编发布时间：2022-01-06 14:16

N6 -methyl-adenosine (m6 A) is the most common and abundant modification on RNA molecules present in eukaryotes. The m6 A modification is catalyzed by a methyltransferase complex METTL3 and removed by the recently discovered m6 A RNA demethylases FTO and ALKBH5, which catalyze m6 A demethylation in an α-ketoglutarate (α-KG)- and Fe2+-dependent manner. It was shown that METTL3, FTO and ALKBH5 play important roles in many biological processes, ranging from development and metabolism to fertility. m6 A accounts for more than 80% of all RNA base methylations and exists in various species. m6 A is mainly distributed in mRNA and also occurs in noncoding RNA such as tRNA, rRNA and snRNA. The relative abundance of m6 A in mRNA transcripts has been shown to affect RNA metabolism processes such as splicing, nuclear export, translation ability and stability and RNA transcription. Abnormal m6 A methylation levels induced by defects in m6 A RNA methylase and demethylase could lead to dysfunction of RNA and cause disease.

For example, abnormally low levels of m6 A in target mRNAs due to increased FTO activity in patients with FTO mutations, through an as-yet undefined pathway, contributes to the onset of obesity and related diseases. The dynamic and reversible chemical m6 A modification on RNA may also serve as a novel epigenetic marker of profound biological significance. Therefore, more useful information for better understanding of m6 A RNA methylation levels and distribution on RNA transcripts could benefit diagnostics and therapeutics of disease.

m6A RNA Methylation Quantification Kit(Colorimetric)(图1)